This brings the Hue/Saturation dialog box back up and we can change the hair color simply by dragging the Hue slider to something different. We can also re-adjust the saturation of the color with the Saturation color. Since this is an adjustment layer, we're free to make as many changes as we want without worrying about damaging our image. Just for fun, I'll set my Hue slider to 304 to give her hair more of a pink/purple look:
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The resulting cryoprotective gelatine block (containing the brain) was then removed from the mould, trimmed and placed, along with the pins, into 4% PFA 30% sucrose overnight. The pins were then removed and the block was sectioned on a sliding freezing microtome at 50 µm thickness in the sagittal plane. Sections were collected in 0.01% sodium azide PBS solution. For each hemisphere every third section was mounted serially onto gelatine chrome-alum coated slides, stained with haematoxylin neutral red, neutral red or cresyl violet (see below), dehydrated and coverslipped with DPX mounting medium (Scharlau) from xylene. The remaining sections were set apart in two series, one for storage and the other for immunocytochemical staining (see below). 2b1af7f3a8