H Kaur Book Spectroscopy Pdf 94 [PORTABLE]
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Raman spectroscopy (/ˈrɑːmən/) (named after Indian physicist C. V. Raman) is a spectroscopic technique typically used to determine vibrational modes of molecules, although rotational and other low-frequency modes of systems may also be observed.[1] Raman spectroscopy is commonly used in chemistry to provide a structural fingerprint by which molecules can be identified.
Raman spectroscopy relies upon inelastic scattering of photons, known as Raman scattering. A source of monochromatic light, usually from a laser in the visible, near infrared, or near ultraviolet range is used, although X-rays can also be used. The laser light interacts with molecular vibrations, phonons or other excitations in the system, resulting in the energy of the laser photons being shifted up or down. The shift in energy gives information about the vibrational modes in the system. Infrared spectroscopy typically yields similar yet complementary information.
The name "Raman spectroscopy" typically refers to vibrational Raman using laser wavelengths which are not absorbed by the sample. There are many other variations of Raman spectroscopy including surface-enhanced Raman, resonance Raman, tip-enhanced Raman, polarized Raman, stimulated Raman, transmission Raman, spatially-offset Raman, and hyper Raman.
The Raman effect is based on the interaction between the electron cloud of a sample and the external electric field of the monochromatic light, which can create an induced dipole moment within the molecule based on its polarizability. Because the laser light does not excite the molecule there can be no real transition between energy levels.[2] The Raman effect should not be confused with emission (fluorescence or phosphorescence), where a molecule in an excited electronic state emits a photon and returns to the ground electronic state, in many cases to a vibrationally excited state on the ground electronic state potential energy surface. Raman scattering also contrasts with infrared (IR) absorption, where the energy of the absorbed photon matches the difference in energy between the initial and final rovibronic states. The dependence of Raman on the electric dipole-electric dipole polarizability derivative also differs from IR spectroscopy, which depends on the electric dipole moment derivative, the atomic polar tensor (APT). This contrasting feature allows rovibronic transitions that might not be active in IR to be analyzed using Raman spectroscopy, as exemplified by the rule of mutual exclusion in centrosymmetric molecules. Transitions which have large Raman intensities often have weak IR intensities and vice versa. If a bond is strongly polarized, a small change in its length such as that which occurs during a vibration has only a small resultant effect on polarization. Vibrations involving polar bonds (e.g. C-O , N-O , O-H) are therefore, comparatively weak Raman scatterers. Such polarized bonds, however, carry their electrical charges during the vibrational motion, (unless neutralized by symmetry factors), and this results in a larger net dipole moment change during the vibration, producing a strong IR absorption band. Conversely, relatively neutral bonds (e.g. C-C , C-H , C=C) suffer large changes in polarizability during a vibration. However, the dipole moment is not similarly affected such that while vibrations involving predominantly this type of bond are strong Raman scatterers, they are weak in the IR. A third vibrational spectroscopy technique, inelastic incoherent neutron scattering (IINS), can be used to determine the frequencies of vibrations in highly symmetric molecules that may be both IR and Raman inactive. The IINS selection rules, or allowed transitions, differ from those of IR and Raman, so the three techniques are complementary. They all give the same frequency for a given vibrational transition, but the relative intensities provide different information due to the different types of interaction between the molecule and the incoming particles, photons for IR and Raman, and neutrons for IINS.
In the years following its discovery, Raman spectroscopy was used to provide the first catalog of molecular vibrational frequencies. Typically, the sample was held in a long tube and illuminated along its length with a beam of filtered monochromatic light generated by a gas discharge lamp. The photons that were scattered by the sample were collected through an optical flat at the end of the tube. To maximize the sensitivity, the sample was highly concentrated (1 M or more) and relatively large volumes (5 mL or more) were used.
Modern Raman spectroscopy nearly always involves the use of lasers as excitation light sources. Because lasers were not available until more than three decades after the discovery of the effect, Raman and Krishnan used a mercury lamp and photographic plates to record spectra. Early spectra took hours or even days to acquire due to weak light sources, poor sensitivity of the detectors and the weak Raman scattering cross-sections of most materials. Various colored filters and chemical solutions were used to select certain wavelength regions for excitation and detection but the photographic spectra were still dominated by a broad center line corresponding to Rayleigh scattering of the excitation source.[8]
Technological advances have made Raman spectroscopy much more sensitive, particularly since the 1980s. The most common modern detectors are now charge-coupled devices (CCDs). Photodiode arrays and photomultiplier tubes were common prior to the adoption of CCDs. The advent of reliable, stable, inexpensive lasers with narrow bandwidths has also had an impact.[9]
Raman spectroscopy requires a light source such as a laser. The resolution of the spectrum relies on the bandwidth of the laser source used.[10] Generally shorter wavelength lasers give stronger Raman scattering due to the ν4 increase in Raman scattering cross-sections, but issues with sample degradation or fluorescence may result.[9]
Continuous wave lasers are most common for normal Raman spectroscopy, but pulsed lasers may also be used. These often have wider bandwidths than their CW counterparts but are very useful for other forms of Raman spectroscopy such as transient, time-resolved and resonance Raman.[10][11]
In solid state chemistry and the bio-pharmaceutical industry, Raman spectroscopy can be used to not only identify active pharmaceutical ingredients (APIs), but to identify their polymorphic forms, if more than one exist. For example, the drug Cayston (aztreonam), marketed by Gilead Sciences for cystic fibrosis,[19] can be identified and characterized by IR and Raman spectroscopy. Using the correct polymorphic form in bio-pharmaceutical formulations is critical, since different forms have different physical properties, like solubility and melting point.
Raman spectroscopy has a wide variety of applications in biology and medicine. It has helped confirm the existence of low-frequency phonons[20] in proteins and DNA,[21][22][23][24] promoting studies of low-frequency collective motion in proteins and DNA and their biological functions.[25][26] Raman reporter molecules with olefin or alkyne moieties are being developed for tissue imaging with SERS-labeled antibodies.[27] Raman spectroscopy has also been used as a noninvasive technique for real-time, in situ biochemical characterization of wounds. Multivariate analysis of Raman spectra has enabled development of a quantitative measure for wound healing progress.[28] Spatially offset Raman spectroscopy (SORS), which is less sensitive to surface layers than conventional Raman, can be used to discover counterfeit drugs without opening their packaging, and to non-invasively study biological tissue.[29] A huge reason why Raman spectroscopy is so useful in biological applications is because its results often do not face interference from water molecules, due to the fact that they have permanent dipole moments, and as a result, the Raman scattering cannot be picked up on. This is a large advantage, specifically in biological applications.[30] Raman spectroscopy also has a wide usage for studying biominerals.[31] Lastly, Raman gas analyzers have many practical applications, including real-time monitoring of anesthetic and respiratory gas mixtures during surgery.
Raman spectroscopy is an efficient and non-destructive way to investigate works of art and cultural heritage artifacts, in part because it is a non-invasive process which can be applied in situ.[36] It can be used to analyze the corrosion products on the surfaces of artifacts (statues, pottery, etc.), which can lend insight into the corrosive environments experienced by the artifacts. The resulting spectra can also be compared to the spectra of surfaces that are cleaned or intentionally corroded, which can aid in determining the authenticity of valuable historical artifacts.[37]
In addition to paintings and artifacts, Raman spectroscopy can be used to investigate the chemical composition of historical documents (such as the Book of Kells), which can provide insight about the social and economic conditions when they were created.[41] It also offers a noninvasive way to determine the best method of preservation or conservation of such cultural heritage artifacts, by providing insight into the causes behind deterioration.[42]
Raman spectroscopy offers several advantages for microscopic analysis. Since it is a light scattering technique, specimens do not need to be fixed or sectioned. Raman spectra can be collected from a very small volume (< 1 µm in diameter, < 10 µm in depth); these spectra allow the identification of species present in that volume.[44] Water does not generally interfere with Raman spectral analysis. Thus, Raman spectroscopy is suitable for the microscopic examination of minerals, materials such as polymers and ceramics, cells, proteins and forensic trace evidence. A Raman microscope begins with a standard optical microscope, and adds an excitation laser, a monochromator or polychromator, and a sensitive detector (such as a charge-coupled device (CCD), or photomultiplier tube (PMT)). FT-Raman has also been used with microscopes, typically in combination with near-infrared (NIR) laser excitation. Ultraviolet microscopes and UV enhanced optics must be used when a UV laser source is used for Raman microspectroscopy. 2b1af7f3a8
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