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On successful completion of the module you should be able to: distinguish between traditional and recent types of cost management systems; distinguish between different purposes of cost management systems; discuss principles of cost management systems design, development, implementation, and use; discuss the effectiveness of cost management systems for different control objectives in decentralised organisations; evaluate the suitability of costing systems in relation to organisational context; speculate about the future of cost management systems; critically analyse case studies; construct robust narratives, combining strategic business considerations and cost management system detail; prepare cost management information fit for management decision-making. Building on ACCT 3251: Intermediate Management Accounting, students explore the integrative and interdisciplinary role of management accounting and its contribution to the strategic management process. Students discuss the provision of quantitative and non-quantitative information for planning, control, and decision making. Topics include costing systems; allocating costs and revenues including support service costs, fixed costs, joint product costs, bundled product revenues, and customer profitability; management information systems; the importance of budgeting; the importance of variance analysis; capital budgeting and investment decision making; quality issues and supply chain strategies; transfer pricing; performance measurement; compensation issues; and strategic processes and balanced scorecards. In this course students will use management accounting information for strategic and operational decision-making. Course topics include management control systems, the performance of individual managers, and strategies for avoiding sub-optimal managerial decisions. This course qualifies as a prerequisite for entrance into the Chartered Professional Accountant (CPA) Professional Education Program (PEP) when completed in conjunction with ACCG 6210 Management Accounting - Comprehensive. This module aims to provide students with an appreciation and understanding of the complexities involved in the design of management control systems. This is achieved by firstly introducing students to the need for management control and the causes of management control problems. Students will then be familiarised with the different types of controls and their characteristics, before being presented with the criteria for the evaluation of the design of management control systems. The interplay and interconnections between the components within the package of controls will be highlighted and the consideration of unintended consequences brought to the fore. Students will be equipped with knowledge of contemporary performance measurement and management frameworks used within for-profit and not-for-profit organisations. The effects of situational contingencies on the design of management control systems will be discussed. Presents advanced knowledge and skills in management accounting for support in organisational strategic decision-making. Students will develop and apply analytical and problem-solving skills to provide advice on issues associated with strategy development, implementation and monitoring. Using case studies, students will tackle contemporary issues such as sustainable management practices, effective corporate social responsibility and managing multi-national operations. The course follows on from BUACC2613 Management Accounting 1. BUACC3714 is a comprehensive course that addresses the key influences on management accounting systems and the changing role of management accountants. It enables students to develop an understanding of the theoretical and practical aspects of management accounting. It covers both the conventional and the contemporary approaches to designing management accounting systems. The conventional approaches include product costing systems, budgeting and the use of standard costs. The contemporary approaches covered in this course include and integration of both financial and non-financial measures into a strategic performance measurement system, sustainability and corporate social responsibility. The course also includes an examination of incremental analysis and capital expenditure decisions. Each topic is introduced by a critical review of key theoretical concepts that are then applied to practical settings. This text provides leading-edge treatment of innovative management accounting issues used by major companies throughout the world. Takes a systematic management- oriented approach to advanced management topics. 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The overall aim of the course is to enable the students to evaluate and construct management accounting solutions from a theoretical point of view and to enable the students to read and understand contemporary accounting research articles. The additional main objective of this course is to give the student a deeper understanding of management accounting and its use in specific contexts. The modern firm is characterized by complex production systems and use of advanced knowledge. This perception of the modern firm is central to the course. Consequently, the interactions among the constituents of the firm, the economic uncertainty, the institutional setup and the organizational form are discussed throughout the course. Furthermore, management control in firms with delegated expertise is analyzed. Thus, strategic use of private information is essential for the construction and development of the management accounting system. The course will use a variety of methodologies in order to enhance the choice of accounting systems. This paper aims to develop skills and critical ability in management accounting. It will broaden the knowledge of participants by examining the diverse perspectives and approaches evident in the subject of management accounting. ACCT 491 - Advanced Management AccountingCourse Description: This course develops a conceptual understanding of the role of management accounting information. Students learn to perform analyses to support managerial decisions, design and implement effective management control systems, and develop an awareness of the moral responsibilities of management accountants. Topics include revenue variance analysis, quality as a competitive tool, transfer pricing, corporate sustainability and the balanced scorecard. Students will further develop their spreadsheet and presentation skills using advanced spreadsheet and presentation programs.3 Credits Technical Requirements Students should have completed intermediate-level coursework in the following area(s) prior to registering for this advanced-level course: ACCT 4220 - Advanced Management AccountingCourse Description: This course develops a conceptual understanding of the role of management accounting information. Students learn to perform analyses to support managerial decisions, design and implement effective management control systems, and develop an awareness of the moral responsibilities of management accountants. Topics include revenue variance analysis, quality as a competitive tool, transfer pricing, corporate sustainability and the balanced scorecard. Students will further develop their spreadsheet and presentation skills using advanced spreadsheet and presentation programs.3 Credits Prerequisite(s): ACCT 3120 2015 - 2020, Southern Alberta Institute of Technology (SAIT). All Rights Reserved. ACC 4300 - Advanced Management and Cost Accounting.(3-0-3) On Demand. The emphasis of the course is on the way management accounting data influence decisions within the organization. Course examines cost planning, cost control, decision making using accounting data, and performance evaluation. 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All rights reserved. AllStickerPrinting is an online sticker printing company that offers custom paper and vinyl stickers. We offer the cheapest solution to promote your business with customized stickers. Several finishing options are available for additional protection, plus, full color printing for the sticker to stand out. You may also order for special events like birthdays, weddings, and similar events. We ensure that we only use high-quality materials and adhesives. We provide free proofing and shipping, quick turnaround guaranteed. AllStickerPrinting understands the importance of quality. To ensure the quality of materials before placing bulk orders, you can request a sample pack from us. You may initially order 100 custom stickers through our paid custom sample pack. Buy online: The Department of Natural Resources has the option to purchase certain annual Wisconsin State Park vehicle admission stickers online. Annual Wisconsin Resident, Annual Wisconsin Resident Senior and Non-resident Annual stickers can be purchased online. Additionally, one Reduced Rate Annual sticker (for a vehicle registered to the same household address) can be purchased online at the same time as a full-price sticker. An admission sticker or admission sticker package can be purchased one at a time through the online service. Annual state trail passes and daily admission stickers and passes are not currently available for purchase online. A combination of stickers/passes can be purchased in one transaction at a state park property. Many companies and organizations purchase Wisconsin state park annual vehicle admission stickers for their members. If you are interested in purchasing a bulk order of annual stickers on behalf of yourself or your organization, please fill out this questionnaire. A State Park System representative will then work with you to process your bulk order. Orders take about two weeks to process/ship. Please remember, that there is no discount for bulk orders, all stickers are at the same rate regardless of quantity. Reduced rate vehicle admission stickers are available for households with additional vehicles registered to the same address. One reduced-rate annual vehicle admission sticker can be purchased online at the same time as a full-price annual sticker. Reduced rate stickers are also sold at individual park properties if you are purchasing more than one reduced rate sticker for your household. Telephone orders: Telephone orders are not sent a receipt via email. Visitors are encouraged to purchase admission stickers online or at individual properties via self-registration stations, electronic sales kiosks or drive-up window service if they are planning a visit to a state park property within 30 days from the date of purchase. For any questions about your telephone purchase, please contact the property from where you ordered your stickers/passes. The same vehicle admission rules apply to rental cars. Admission stickers must correspond to the license plate of the vehicle (a rental vehicle with out-of-state license plates must purchase a non-resident admission sticker). Per state statute, an admission sticker is non-transferable and must be affixed to the front windshield by its own adhesive to be valid. If you have not received your sticker yet, follow the guidelines above. Yes, a vehicle admission sticker is required for any motor vehicles brought into the property, including campgrounds. A camper without an annual sticker will need to buy one daily sticker for each night of camping. Free daily stickers are available for vehicles towed or carried into the property by a vehicle with a purchased sticker. 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Multiplayer support with up to four players was added in a patch. The game received a generally positive reception, with reviewers praising the graphics and humour in particular. Theme Hospital was a commercial success, selling over 4 million copies worldwide, and was ported to the PlayStation in 1998. A Sega Saturn version was in development, but cancelled. The game was re-released on GOG.com in 2012 and Origin in 2015, and the PlayStation version was released on the PlayStation Network in Europe in 2008, Japan in 2009, and North America in 2010. Revival attempts have been made with the development of open-source remakes such as CorsixTH. Diagnosis and treatment cost patients money, and the player can change hospital policy, including the amount of diagnosis patients require. This can be set to over 100 per cent to force patients to have further unnecessary diagnoses. Other policies include when staff can go on breaks and whether they can leave rooms,[18] and loans can be taken out.[19] From time to time, events such as emergencies (in which patients arrive and must be cured within a time limit or they will die), and epidemics (in which a disease spreads rapidly) occur. During the latter, the player can attempt to cover it up by curing all affected patients before a health inspector turns up. If the player fails, they are fined and must face a damaged reputation,[20] a statistic that shows how well the hospital is doing and affects the flow of patients.[21] VIPs may also occasionally ask to tour the hospital; if impressed, the player is granted a cash bonus, a reputation increase, or both.[22] There is an advisor who keeps the player informed about what is going on.[23] Rats may infest the hospital, and the player is able to shoot them with the cursor.[24] Although the player has no direct control over the patients, they have some influence over whether to evict them from the hospital and in determining what to do with them when given a choice by the staff. The player can pick up any staff member in the building and move them (mainly to reassign them to another room or send them to get rest), or dismiss them if they argue about pay or are no longer required.[25] The player may force patients to take a chance at a cure for their suspected disease before diagnosis is complete (at the risk of killing the patient),[26] and rearrange queues.[27] Each level has set goals in the fields of financial attainment, hospital reputation, patients cured, and hospital value.[4] Holding negative funds or allowing sufficient patients to die will bring about losing requirements. When the goals have been met the player has the option to move on to a new, more elaborate hospital with tougher winning conditions and more diseases present, or stick with their current one.[30] If enough rats have been shot, the player is taken to a special level whose goal is to shoot as many as possible.[24] A patch was released that fixed bugs and added support for Serial Cable, Modem, and IPX/SPX network gaming (with up to four players[31]) in the Windows version, and a difficulty setting.[4][32][33] In multiplayer, each player has a colour, which patients will wear to distinguish from those of other players. \"Litter Bombs\" are featured, allowing players to spread litter around other players' hospitals, while staff members can be persuaded to work for other players, and there are \"Mini Missions\", with instructions for players to perform. Players are also able to chat with each other during a game.[32] Theme Hospital took over two years to develop.[34] The idea came from Peter Molyneux and journalist James Leach, who explored the possibility of other Theme games while discussing Theme Park. Leach suggested a hospital, and Molyneux was enthusiastic about the idea. Development began when the designer Mark Webley selected Theme Hospital from a list of possible games put together by Molyneux, and Webley, along with the artist Gary Carr, visited the Royal Surrey County Hospital, Great Ormond Street Hospital, and Frimley Park Hospital for research and inspiration.[5][35] Carr was initially displeased at the prospect of working on Theme Hospital: he had previously left Bullfrog to work for The Bitmap Brothers because he did not have faith in Theme Park. He chose to return based on his belief that he would work on Dungeon Keeper, which he was eager to do. Initially, Theme Hospital was to feature real diseases, but these were replaced with fictional, comedic afflictions as it was decided that the game's maladies should not be too realistic:[5] the team were faced with the dilemma of having to make the hospital fun and interesting, as Webley stated that theme parks are \"colourful\" compared to hospitals, and Carr believed that hospital rooms looked very similar and that it was hard to tell what each machine does.[36] It was during a hospital visit (after witnessing an operation that they were ordered out of because they were distracting the surgeon[5][37]) that they came up with the idea of using made-up illnesses and cures: Carr considered death \"quite sad\" and believed that illness was not a popular subject.[36] He stated that using made-up illnesses gave them \"creative freedom\", which he believed was essential.[36] Webley said that Carr was concerned that using real diseases might be considered distasteful.[37] According to Carr, the team were aiming for humour rather than gore.[38] During the initial stages of programming, Bullfrog hired James Leach, who wrote the game's text and came up with the diseases. Molyneux was not directly involved in the game's production as he was then working on Dungeon Keeper.[5] The game deviates from the NHS model; Webley and Carr decided that players should think of the hospital as a business, with profit highly important. The team, who worked in offices near the Royal Surrey County Hospital, drew some inspirations from Theme Park and borrowed a great deal of code.[5] Webley explained that this was an \"obvious starting point\" as he had been a programmer on Theme Park, and that the systems had been rewritten by the time Theme Hospital was complete.[35] A program which facilitated animation was built upon by Webley, who dubbed it the Complex Engine. There were not many meetings, and Webley took the team to the pub weekly with a list of tasks to perform. Webley explained that the way the team worked meant that only they could perform their assigned duties otherwise they would not get done, effectively giving members ownership of their parts of the game.[5] It was during one such meeting that the team realised the game was complete.[37] The disease called King Complex was to be called Elvis Impersonator, but Elvis's estate owned the rights. Bloaty Head was based on an allergic reaction Molyneux had, when his face became an \"alarming size\".[5] Theme Hospital was released on 28 March 1997 (although it was previously due for release in November 1996[44]),[5][41][1] and is part of Bullfrog's Designer Series.[45][46] The game attracted some controversy from NHS managers, the Houses of Parliament, and The Daily Telegraph, who published an article criticising the game as \"sick\" and said that the British Medical Association was using it to train management staff.[5][37] The NHS argued that the game mocked hospital management. Webley was invited to appear on a local radio station; he did not consider the game to make light of real-world health management because it featured fictional diseases rather than real ones. Theme Hospital was a commercial success, which surprised Bullfrog and Electronic Arts. It repeatedly appeared in the top five of budget charts and sold over 4 million copies. In 2014, Molyneux stated that he wanted the game to make a comeback, and described Theme Hospital as \"a great game that I and many people remember\".[5] Another reviewer who liked the graphics was Dawn Jepsen of Computer Gaming World, who also praised the humour, especially the \"wackiness\" of the illnesses, and described the game as \"delightful and absorbing\".[4] The humour was also approved by a reviewer of Jeuxvideo.com, who, like other reviewers, lauded the graphics, by describing them as colourful.[73] Next Generation's reviewer echoed others' views about the humour, describing it as something they had come to expect from Bullfrog, but criticised the lack of multiplayer support.[76] The humour was again praised by a reviewer of PC Power, who said that the ailments enabled Bullfrog to have \"hilarious\" cures. The \"thoroughly engrossing\" gameplay, and the graphics were also complimented: it was stated that the bright colours gave hospitals a \"cheerful look\". Theme Hospital was described as a \"fantastic title\".[78] Other comments from critics included Edge's reviewer's compliments of the balance of patients and illnesses against the player's resources, and his belief that the game is a refinement of its genre,[70] and PC Zone's reviewers commendation of the detail: it was remarked that it gets players \"helplessly immersed\", and the game was awarded the PC Zone Classic accolade.[75] Éric Ernaux of Génération 4 said the quality is \"remarkable for a game of its genre\".[79] 59ce067264 https://www.recovery.church/group/recovery-church-group/discussion/d44290b3-c81b-4a6e-b13e-05f4bec6ea59

30 : Sparks Fly For Magnemite Download File ->>->>->> https://urlca.com/2tkYn4 Upon arriving at the polluted industrial town of Gringey City, Ash and his friends notice that Pikachu has become faint and sparks are coming out of his cheeks. They subsequently rush to the Pokémon Center as they are watched by a shadowy Pokémon, as well as Meowth. Jessie and James begin to recite their motto, though a foul stench forces them to stop. Meowth, who is inside his own protective container, gives them scuba suits to wear, and says he will stay there to pump oxygen and fresh air to them. He instructs them to dive into the water and swim through the pipes to the Pokémon Center, but they refuse. Meowth responds by pushing a button that delivers Jessie and James an electric shock, and they reluctantly enter the sludge-filled sewer waters. At the Pokémon Center, Ash finds an uncharacteristically apathetic Nurse Joy, who dismisses Pikachu's condition as a cold. When Ash yells at her for not being very helpful, she explains that the sparks coming out of Pikachu's cheeks are an early symptom of a cold in Electric rodent Pokémon. She suggests that they leave Pikachu there for the night, and insists that he will be fine in the morning. Ash, Misty, and Brock arrive in Gringey City where everything is dark, highly polluted, and gloomy. Pikachu isn't feeling well, his face is red and sparks are coming out of his cheeks. Ash rushes Pikachu to the Pokémon Center while a shadowy Pokémon watches him. At the Pokémon Center, Ash rings a bell on the front desk and Nurse Joy comes out. It's late at night and she is very tired. She says Pikachu has a cold. Ash yells at her for not being helpful and Nurse Joy explains that the sparks coming from Pikachu is an early sign of a cold in electric mouse Pokémon. She suggests that Ash and his friends leave Pikachu there for the night and come back for him in the morning; by then, Pikachu will be better. 59ce067264 https://www.maislingua.com.br/forum/conversation-club/where-to-buy-a-car-from-a-private-seller

Why Did I Get Married 2 Full Movie Click Here ===> https://cinurl.com/2tkXuD Elsewhere, at Sheila's request, Mike agrees to help find Troy a job. Angela lectures Dianne and Sheila about how all men cheat. Patricia changes the locks and catches Gavin, Terry, and Marcus moving Gavin's things out, then learns Gavin has taken all their money, including her book money; enraged, Patricia trashes the house with his golf clubs. Angela comes home early to catch Marcus cheating and finds a couple in her bed, but after shooting up the room, she notices it was just the gardener and the maid having sex. Terry finally confronts Dianne about her infidelity; she reveals that she has been having an emotional affair and begs for forgiveness. Marcus and Angela fight, then reconcile, but only to fight again after Angela discovers Marcus has another phone. Troy arrives at Mike's apartment after finding out Mike got him his police job. After finding Sheila there, he angrily attacks Mike. Sheila tearfully confesses that she has been taking Mike to chemotherapy; she tries to apologize for being dishonest, but he leaves her. One year later, as Patricia exits a university building she is approached by a colleague. She tells Patricia that she knows someone who wants to meet her, a philanthropist, but Patricia refuses. The professor goes on to tell her that she can at least say hi because the university needs funds. The man (Dwayne \"The Rock\" Johnson) tells her that her books have helped in his grieving process (divorce) and invites her to have coffee. The movie ends with Patricia smiling at him. Wesley Morris of The Boston Globe gave the film 2 out of 4 stars, claiming: \"If Perry's cinematic vision remains less than 20/20, his sagacity gets stronger by the movie.\"[9] Lisa Schwarzbaum of Entertainment Weekly graded the film a D, writing in her review: \"It's a contradiction in terms to think of the phenomenally successful, prolific entertainment showman Tyler Perry as lazy, but there's no other description for this particular product: Terribly shot and crudely assembled.\"[10] Scott Wilson concurred, stating: \"Why Did I Get Married Too lacks the underlying goodwill of some of his better work and plays like a gift wrapped present to his detractors.\" Wilson graded the movie 1.5/5 stars. Why Did I Get Married Too grossed a total of $30.2 million in its opening weekend placing second behind Clash of the Titans. Its opening weekend gross makes it the third highest opening for films created by Tyler Perry.[11] As of June 6, 2010 the movie has grossed over $60 million domestically.[12] Four couples, who are also best friends since college, converge in a house in the mountains for a week-long retreat. This ritual of sorts aims to help them work out their marital problems and ask the question \"Why did I get married\". Though the couples have committed to being physically present for the week, some of them have not been emotionally present in their respective marriages for quite some time. The week is not planned out in a well-programmed sequence, so the events unfold somewhat spontaneously, beginning with their \"adventures\" in getting up to the mountain retreat. Some time later, the couples converge at a gala celebration for an award recently received by Patricia. She, Dianne, and Angela are elated when Sheila arrives, re-introduces Troy as her new husband, and has successfully lost weight with his help. Troy meets up with the husbands, who are also gladly surprised by the news of his and Sheila's marriage. Although Mike's still with Trina, he tries to weasel his way back into his ex-wife's good graces, but she turns him down, telling him to go enjoy Trina. Patricia encloses a confession of her love for Gavin and a message of loving, respecting, and trusting God in her acceptance speech. Review aggregation website Rotten Tomatoes gives the film a score of 48% based on 42 reviews, with an average rating of 5.50/10. The website's critics consensus reads: \"Despite some poignant observations on modern marriages, Why Did I Get Married is too preachy and melodramatic.\"[5] On Metacritic, the film had an average score of 54/100 based on reviews from 12 critics.[6] CinemaScore polls reported that the average grade moviegoers gave the film was an \"A+\" on an A+ to F scale.[7][8] Four couples reunite for their annual vacation in order to socialize and to spend time analyzing their marriages. Their intimate week in the Bahamas is disrupted by the arrival of an ex-husband determined to win back his recently remarried wife. The movie started with the four couples going for a retreat to Bahamas. At first, Patricia and Gavin were perceived to have been in the best marriage but at the end they broke up and Gavin died in an accident (IMDb 1). This movie is very appropriate for the married people and even young teens. The movie brings out the theme of marriage and finances clearly. It is interesting and full of suspense as the director is able to bring out many conflicts in marriages within a short time.Dialogue between the characters is intensively used. However, this movie only targets the adults and only includes black Americans. Critics have claimed that the director did not take into account the economic recession and that the movie did not use the seting of exotic Bahamas appropriately (Tsai 1). An analysis of this movie revealed that it effectively showed effects of infidelity and importance of communication between a husband and a wife. The movie started when four couples who had been friends for a long time went for a vacation in Bahamas. The movie started with Terry dribbling a ball in their living room when their young daughter started to describe what divorce was to her brother. The second half of the movie was when the couples went back to their homes. Their marriages fault lines were seen in this scene. Each couple was going through a challenge and Gavin lost his life at the end while driving after a quarrel with Patricia (Hale 1). The movie shows the struggles wives and husbands go through. The movie is said to have a soap opera style especially with Sheila who is very emotional (Tsai 1). She had worked so hard for her second marriage with Troy to succeed. Troy on the other hand was offended because she exposed his financial struggles. He felt like others were undermining him and turned his anger to his wife Sheila (Perry 1). There was suspense throughout the movie, for example, when Terry moved out from his home and went to live in a new house. Dianne was desperate to get her husband only to find him with another girl. Terry mentioned her name and stated that she was pregnant. Patricia was the protagonist in this movie. She was a commander in their marriage with Gavin. She was a psychiatrist and more theoretical than practical. It was ironical that she advised other couples and even wrote a book about marriage whereas her marriage was going through hell. All the characters acted in the earlier movie, Why Did I Get Married where they played the same roles as in this movie. However at that time Troy was a Sherriff. This family movie has the same theme of marriage with Not Easily Broken which was directed by Bill Duke. The camera is used to effectively in this movie by use of camera techniques. There are numerous close up shots which are used to create interest and suspense. They give details of the characters. There are also several nature shots which help to give details of the environment in Bahamas. Tyler Perry is a film institution like no other today. With 2005's Diary of a Mad Black Woman, the writer/director/producer/actor took the brand of entertainment he developed on the stages of Atlanta (and other stops on the urban theater circuit) and turned it into its own class of cinema. The box office success of that film paved the way for other adaptations of his plays, all of which he has scripted, produced, and directed and most of which he has also acted in.Five years since his big screen debut, Perry is about to release his tenth signature film, this one (For Colored Girls, recently dropping the rest of its original title: Who Have Considered Suicide When the Rainbow is Enuf) his first adaptation of someone else's play and his first to put itself into award season consideration. In addition to that, Perry has lent his name and support to multiple Oscar winner Precious, has two sitcoms currently running on TBS, and even made a cameo in last year's Star Trek film. Not bad for someone operating almost exclusively in PG-13-rated black family stories, a domain that practically didn't exist before him. Tyler Perry's Why Did I Get Married Too becomes the first official sequel in Perry's filmmaking canon, directly following 2007's Why Did I Get Married. The original performed better than most of his reliably profitable output (three of which rank among distributor Lionsgate's 10 all-time highest-grossing releases). If like me, you didn't see that one, it told the story of four troubled married couples on a retreat (all of whom have darker skin than Vince Vaughn and Jason Bateman) in the mountains of Colorado.All four couples are back, although one had divorced and the woman remarried last time. To everyone's surprise, the ex-husband shows up at the group's getaway to a large beach house in the Bahamas, creating a tense third wheel situation among the nine adults, who leave their kids at home. The gang is comprised of the following pairs: stable, good-humored Terry (Tyler Perry) and Dianne (Sharon Leal); published psychologist Patricia (Janet Jackson, first by billing but probably last by screentime) and Gavin (Malik Yoba); the always-bickering Angela (Tasha Smith) and Marcus (Michael Jai White); and newlywed new parents Sheila (Jill Scott) and Troy (Lamman Rucker). Sheila's abusive ex Mike (Richard T. Jones) is the unwanted party, who nonetheless asserts his right to the timeshare.The movie's first hour is spent in the Bahamas. The second returns everyone back to Atlanta, where new problems emerge and intensify. Probably the biggest among them is that the group's longtime model for marriage, Patricia and Gavin, are divorcing, a process which only starts civilly. The distrustful Angela never stops screaming, making the password to Marcus' cell phone her biggest bone to pick. Terry becomes suspicious that Dianne is having an affair of her own. And Mike's uncharacteristic remorsefulness troubles Sheila and threatens Troy, who's struggling to find a job upon relocation. Why Did I Get Married Too is a rather curious film. It has utter disregard for cinema's standards in pacing and tone. The smaller of the two significant issues is the pacing; the movie runs a full two hours with end credits, which is longer than most earthly, contemporary human interaction pictures. There isn't an abundance of content to justify the length; instead, you spot flabby, aimless scenes throughout, several of which have a DVD deleted scenes section feel to them.The wildly varying tone is tougher to wrap your head around. One moment, the movie is being silly; sports commentator Marcus speaks in football analogies, Angela gets covered in human ashes, a flamboyant male dancer pops out of pretend birthday cake at a professional workplace. In the very next breath, the movie opts for ridiculous seriousness; one husband gets drunk and violent, an elderly couple (Louis Gossett, Jr. and Cicely Tyson) is supposed to move us with their \"why did I get married\" stories, a lead character dies, and another is diagnosed with cancer. It's so ludicrously erratic. Among the divergent approaches, the movie is much more comfortable with the comedy, but it seems to have no idea of that, as it keeps striving for poignancy with even less success than the loud, grating adultery \"humor.\"Though the results are increasingly unpleasant, you can't entirely write off the film, as the voters of IMDb do when they rate Perry's movies low enough to flirt with entering the Bottom 100 list. The bachelor writer/director/star seems sincere in his belief for the institution of marriage and well-intentioned in exploring the issues that divide so many unions. But the world of his upper class characters seems so removed from reality and utterly unable to handle any topic with tact and reason. It's sort of refreshing that you don't mistake the rhythms and personalities of Perry's dramedies for those of any other contemporary film. But said rhythms and personalities are so tough to warm to or even comprehend that the only praise you can offer the creator is for providing representation and stories for groups that Hollywood otherwise ignores. As such, you wish the only person making these kinds of movies was able to treat and serve his people better.Commercially, Married Too was a clear success, earning $60 million on a $20 million budget. Perry's films perform strongly on a crazily consistent basis. The majority have grossed $50-$60 million in their nearly U.S.-only release of 2,000 theaters. Should you fear that For Colored Girls will bid farewell to the Tyler Perry that people know and love, know that Tyler Perry's Madea's Big Happy Family is set to open in just six months. 59ce067264 https://www.haveninc.net/forum/welcome-to-the-forum/s2e6-forever-plaid

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The player controls each of the four Spider-Men as they attempt to retrieve the tablet fragments from villains within their respective dimensions, who now possess new abilities granted by the tablet. There are several drastic gameplay differences from a Spider-Man to another. For example, the Spider-Man Noir levels require players to hide in the shadows and take down enemies stealthily, whereas the Ultimate Spider-Man levels put more emphasis on combat and often have the player fighting large crowds of enemies; this is possible due this version of Spider-Man being provided with the symbiote black suit by Madame Web that increases his strength. The Spider-Man 2099 levels feature unique free falling segments and a bullet time mechanic, which allows players to slow down time to dodge incoming projectiles.[6][9] The Nintendo DS version of Shattered Dimensions is a side-scrolling brawler with little gameplay difference between the multiple iterations of Spider-Man. This version also omits Ultimate Spider-Man, and features less villains: Electro and the Tinkerer for the Amazing Spider-Man; Boomerang and Calypso for Spider-Man Noir; and Vulture and Silvermane for Spider-Man 2099. Not all the moves are available at the beginning, and can be unlocked as players progress through the game.[10] While most of the action is played with pad and button controls, the touchscreen is used to play a mini-game in between levels to help Spider-Man use the Tablet to travel between dimensions.[11] Several pre-order bonuses were offered for the PlayStation 3 and Xbox 360 versions prior to release. Players who pre-ordered at GameStop received a Cosmic Spider-Man pack, which provided a Cosmic Spider-Man outfit for each Spider-Man.[20] It was later included as a free bonus on the PC version, along with some minor fixes, which were not patched on the console versions. The Cosmic Spider-Man pack was later publicly released as downloadable content for the Xbox 360 on October 28,[21] and for the PlayStation 3 on November 2, 2010.[22][23]Pre-orders via Amazon.com included early access to Spider-Man 2099's Iron Spider costume and a book containing concept art for the game.[24] Kmart pre-orders offered early access to Amazing Spider-Man's Scarlet Spider costume, while Best Buy included early access to the Negative Zone costume for Spider-Man Noir.[25] Wal-Mart offered a 20-page comic book for online pre-orders of the game.[26] All the three costumes can otherwise be unlocked with cheat codes on copies of the non-DS versions of the game.[27] IGN gave the PlayStation 3 and Xbox 360 versions eight out of ten and said, \"Spider-Man: Shattered Dimensions has a lot of great things going for it\",[43] but scored the Wii version half a point lower despite being nearly identical to these two versions due to that version lacking a button to center the camera.[45] Game Informer awarded it 8.5 out of 10 and said it \"delivers a non-stop action ride with something new around every corner\".[35] GamesRadar gave it three-and-a-half stars out of five and said \"Shattered Dimensions is a good game, but it fell short of an Ultimate Marvel Team-Up\".[52] GamePro gave the Xbox 360 version four and a half out of five stars and said, \"thankfully, [Spider-Man's] latest adventure, Shattered Dimensions, is one of his best yet, offering four unique worlds, classic Spidey action, and an engaging story that puts it near the top of the franchise both in terms of quality and impact\".[36] X-Play gave the game four stars out of five and said it \"streamlines the usual trappings of the genre to focus on combat and personality. It's true that the linear gameplay and old-school combat sensibilities don't put it on par with Arkham Asylum, but the game succeeds regardless. It's fun, fast-paced, and long enough to make it well worth checking out\".[53] 1UP.com gave it a B and stated, \"the change in combat options is a welcome addition, I just wish that the story had delivered on the comic legacy of one of Marvel's more enduring heroes\".[54] Spider Man Shattered Dimensions is based on the Marvel Comics super hero Spider Man. In this game the player will have to control four different version of Spider Man and each one of the versions are originating from different universe. The four versions of Spider Man are from Amazing, Noir, 2099 and Ultimate universes. In every universe Spider Man has got a different and unique fighting styles. Amazing Spider Man uses a combo of melee and web attacks. Spider Man Noir uses stealth and timing for defeating the enemies in a fight silently. Spider Man 2099 uses the acrobatics as well as the advanced abilities of his suit ina fight. Ultimate Spider Man uses the powers of the symbiote suit to defeat the enemies. Spider Man Shattered Dimensions has got amazing graphics which you will surely love. You can also download Spider Man 3. In the game Spider-Man: Shattered Dimensions, you play as four different versions of Spider-Man. The game begins when a mystical artifact called the Tablet of Order and Chaos is broken into four pieces and spread over four dimensions, causing problems with all of reality. When a sorcerer tells Spider-Man of the situation, Spider-Man then is forced to get help from other versions of himself to find and reunite the pieces of the tablet. On the Console versions of the game, the story is divided into three \"acts,\" which get progressively more difficult. Each act contains 4 stages [one for each Spider-Man], and the player is able to freely choose which order the act's stages are done in [the only exception is Kraven's stage, which the player is forced to do first in the first act. After that, the remaining three stages can be done in any order]. When all four stages in an act are clear, the next Act opens. All shops featured on GG.deals will deliver your game immediately after the payment has been approved. This will be either in the form of direct download or PC key - depending on the store of your choice. After you activate key on a corresponding platform, you will be able to download and play your game for free. If you don't know how to activate the key, check out the tutorials section on the bottom of the page. In this best version of the spider man game spider man edge of time for pc, you can upgrade the system, purchase new fighting moves, increase stats, and unlock alternate costumes those challenges that you will be facing during playing the game. The original spider-man has the ability to move quickly through a laser defense system without hurting enemies and spider man 99 creates a fake simulation of himself to divert the enemy. A similar type of action-adventure game ben 10 game download for pc and play with your friends. 59ce067264 https://www.lydiakapellmd.com/forum/questions-answers/a-mothers-love-apk-download-latest-v-socigames

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Rumors are that Coinbase will allow XRP trading again shortly, but until then, Uphold, Kraken and Coinmama are a couple of your options. First, CoinCodex had a somewhat pessimistic short-term ripple price prediction for 2023. The site said that it thought the price of XRP could drop to $0.4742 by 2 April and could continue its decline to $0.459 by 27 April. Despite that, the site's technical analysis remained bullish, with 24 indicators sending out positive signals compared to just five making bearish ones. The ripple crypto price prediction from DigitalCoinPrice anticipated growth, with it potentially averaging $0.95 in 2023 and $1.21 in 2024. The site made a ripple price prediction for 2025 that suggested it could reach around $1.65, before potentially hitting $4.96 in 2030. Over the years, we've identified eToro as one of the best place to buy XRP today. The platform is secured and very safe. It is one of the first online brokers to enable crypto investing, attracting over 30 million users in the process. In this comprehensive post, we have shared the best and most reliable methods of buying XRP with PayPal using industry-leading brokers, exchanges, and apps. The providers uncovered in this post have been cherry-picked based on noteworthy parameters including but not limited to customer reputation, fees, regulation, UI, and choice of cryptocurrencies. The UI/UX engineering at eToro is mesmerizing and undoubtedly, one of the best in the business. You can complete the registration process or transaction with a minimal number of steps. Also, locating XRP or other cryptocurrencies is easy with handy search features. XRP is one of the most popular cryptocurrencies and can be sold on various exchanges and brokers. Sifting through each option can be time-consuming, so we have listed and compared the best exchanges to convert XRP into cash. Our top reveals of where to sell XRP takes into account the verification process, trading fees, withdrawal fees and overall ease of use. Binance is overall the best place to sell Ripple into cash. The platform is the world's largest crypto exchange by trading volume, supporting over 600 digital assets, including XRP. The exchange offers a variety of ways to liquidate XRP tokens, although the most common methods will be its XRP-to-crypto and XRP-to-fiat trading options. Another option is to sell XRP for another asset like the Tether coin (USDT) for direct trading on the Binance peer-to-peer (P2P) service. XRP can only be sold to Bitcoin, Tether, BUSD, Binance Coin, Ethereum and DAI. The Binance P2P service can be daunting at first glance, but it functions like any other P2P platform. Users can manually navigate the platform to filter merchants based on the crypto assets and payment method preferred. The Binance P2P marketplace supports Zelle, SWIFT, bank transfer and CashApp withdrawal options. A benefit is that XRP holders can choose the exact payment method and fiat currency to receive funds in after selling. A disadvantage with Bitstamp from our testing of the platform is the limited withdrawal options and low trading fees suit large investors only. Moreover, there isn't a P2P marketplace where XRP traders can visit to exchange their tokens and receive their funds in their preferred payment method. Rounding up our list of best places to sell Ripple is Kraken. The platform was launched in 2011 and is one of the top crypto exchanges per trading volume. The digital asset hub is home to over 250 cryptocurrencies, including XRP. There are many wallets that can store XRP. Depending on their need for security and functionality, users can choose the wallet that suits them. Kriptomat exchange is a great place to store your XRP, as it offers a large range of functional options (buying, selling, exchanging for other cryptocurrencies) without ever compromising on security. The currency on which transactions can be carried out across RippleNet is Ripple (XRP). Ripple can be mined and traded. It can also be bought from exchanges and resold at a higher price. The third marketplace for Ripple exists on forex platforms where XRP is listed as a CFD asset, usually in a pairing with the US Dollar. The price of XRP is determined the same way many digital currencies, fiat currencies (such as AUD) and stocks are determined. The listed XRP buy and sell price on Swyftx is set by real-world trade volumes averaged on global cryptocurrency exchanges. This means that the XRP price is influenced by the supply and demand from buyers and sellers worldwide with the current price set at the price of the last trade that took place. The second reason, and the one that is particular to ripple, is that it is currently embroiled in a lawsuit with the US Securities and Exchange Commission (SEC). The SEC argues that XRP is an unregistered security, a claim that Ripple denies. Those learning how to trade XRP need to remember that, unfortunately, the coin rarely seems to be a strong performer when compared with rival cryptocurrencies. Among the top 25 coins based on market capitalisation (excluding Bitcoin), ripple was actually the weakest in the first quarter of 2020. CFDs, spread-betting and futures can often be traded on margin, which effectively means you are borrowing from a broker in order to leverage your position. Once you learn how to trade ripple, it is important to remember that to trade ripple with leverage can be extremely risky. Not only can it magnify profits, it can also generate huge losses. Where are the best places to start trading Liquidity should be one of your top priorities. 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Production companies involved with the series were slated to consist of Lionsgate Television and Feigco Entertainment.[10][11] On June 11, 2020, HBO Max renewed the series for a second season.[12] Janet Hubert, Jordan Rock, and Maya Kazan joined the second season as executive producers.[5] Alongside the initial series announcement, Anna Kendrick was also cast in a lead role.[10] On August 13, 2019, it was reported that Zoë Chao, Sasha Compère and Peter Vack joined the cast in starring roles, with Scoot McNairy joining in a recurring role.[11] On November 5, 2020, William Jackson Harper was cast to headline the second season[4] with Kendrick set to reprise her role of Darby as a guest.[13] On May 3, 2021, it was announced that Jessica Williams and Chris Powell joined the main cast.[5] On July 20, 2021, Punkie Johnson was cast in a starring role while Leslie Bibb, John Earl Jelks, and Arian Moayed were cast in recurring roles for the second season. Kimberly Elise, Ego Nwodim, and Blair Underwood also joined the cast as guest stars for the second season.[6] The following month, Keith David was cast in the role of the season's narrator while Steven Boyer joined the cast in a recurring role.[7][8] Filming had begun by August 2019 in Queens, New York.[14] The final scene of the finale was filmed on March 12, 2020, and production was forced to shut down the next day because of the COVID-19 pandemic. While several more days of filming pick-ups had been planned, they were able to complete the season without those additional shots.[15] The series premiered on May 27, 2020, on HBO Max.[1] In Australia, the series was released on May 27, 2020, on Stan,[17] and public broadcaster BBC acquired television broadcast rights in the United Kingdom (where the series started airing on the broadcaster's main BBC One channel[18]) to the series on September 10, 2020.[19] The series is streaming on Lionsgate Play in India.[20] In the United States, the series was aired on TBS on August 22, 2021.[21]The second season was scheduled to debut on October 28, 2021, with the first three episodes available immediately, follow by three episodes on November 4, and the final four episodes on November 11.[9] The series was removed from HBO Max in December 2022.[3] On Rotten Tomatoes, the first season has an approval rating of 65% based on reviews from 48 critics, with an average rating of 5.8/10. The site's critics consensus reads: \"Love Life first season breezes by on Anna Kendrick's charms, but those looking for a real connection may find its featherweight familiarness frustrating.\"[22] On Metacritic it has a weighted average score of 54 out of 100 based on reviews from 22 critics, indicating \"mixed or average reviews\".[23] On Rotten Tomatoes, the second season holds an approval rating of 100% based on reviews from 12 critics, with an average rating of 7/10. The site's critics consensus states: \"William Jackson Harper's Love Life is easy to get caught up in thanks to the star's charm and this season's thoughtful exploration of a real romantic conundrum.\"[24] We knew from the start that this would be an anthology, with season two focusing on a different love story. How much will Darby remain part of the central narrative How much of the rest of the cast will return and how much will they be involved Speaking to Deadline about stepping down as Love Life's lead, Kendrick said: \"Now that I'm not the lead of the show, I feel like I'm finally allowed to say, 'I love this f***ing show so much. It's so good!' I watched Will's [Jackson Harper] episodes and I'm like, 'Damn, this is just so good.'\" Jackson Harper, who will be filling Kendrick's shoes, added: \"I watched the first season because I'm not really a rom-com guy but it just dives into so much other stuff. It was really like what it's like to be twentysomething in New York and dealing with life in general. It's like a bunch of people in a tiny apartment are all on top of each other and the doors to your room are all see-through. It feels so real.\" \"They don't have to look a certain way ... if the story's there, you're gonna connect with whoever the person is. I think that's why Will [Jackson Harper] is so fantastic this season, that you just instantly lock in with him, and then hopefully people start to think outside the box in all love stories.\" I started Wherever I Look back in 2011 and have aimed to be that friend who loves watching various forms of media and talking about it. So, from bias, strong opinions, and a perspective you may not have thought about, you'll find that in our reviews. Due to coronavirus-forced production shutdowns, Summer 2020 has been an especially soft season. Click through our gallery to see how 18 freshman broadcast television shows -- some acquisitions -- fared in total viewers for their premieres this summer. One of my favorite relationships this season is the one between Marcus and his sister. What did you want to bring to that relationship and when did you realize just what absolute gold that was WILLIAMS: We always wanted to have a light braid between the seasons. We always knew we wanted a little bit of Darby in Season 2, but not so much that it felt artificial or that it was taking any real estate away from Marcus. I think we found a nice balance to just have her be in a shared universe and braid it together, but not, in any way, have it overshadow the new story. Christina Radish is the Senior Entertainment Reporter at Collider. Having worked at Collider for over a decade (since 2009), her primary focus is on film and television interviews with talent both in front of and behind the camera. She is a theme park fanatic, which has lead to covering various land and ride openings, and a huge music fan, for which she judges life by the time before Pearl Jam and the time after. She is also a member of the Critics Choice Association and the Television Critics Association. Love Life, the HBO Max original romantic comedy anthology series, is back with season 2. This time, William Jackson Harper stars as Marcus, a thirtysomething editor who finds himself unexpectedly dating again after his wife divorces him. And narrating his story is Keith David, who takes over for Lesley Manville in season 1. Other guest stars include Maya Kazan, Ego Nwodim, Kimberly Elise, Blair Underwood and Jordan Rock, with season 1 stars Zoë Chao, Peter Vack, Sasha Compére, Nick Thune as well as Kendrick briefly reprising their characters. Love Life season 2 debuts with three episodes on Thursday, Oct. 28. Want to watch more New episodes premiere Nov. 4 and Nov. 11 while season 1 is now streaming on HBO Max. (We may receive an affiliate commission if you subscribe to a service through our links.) Love Life is an anthology series, meaning each season has focused on a different main character. In a potential third chapter, Kendrick hopes to center more diverse experiences. \"[An LGBTQ+ story] was something we talked about when we were running around pitching the show from the jump, so obviously that's something we would be really excited to do,\" she told Variety in October 2021. Love Life on HBO Max is back for season 2 chronicling the life journey of Marcus Evans, played by Emmy Award-nominee and executive producer William Jackson Harper after his marriage unexpectedly implodes. The romantic comedy anthology series kicked off the premiere week of season 2 with a romantic event series supporting Black-owned restaurants across the country in celebration of the upcoming season. From Oct. 26 to 29, couples have the opportunity to dine at eight different restaurants in New York, Atlanta, Detroit, and Los Angeles and enjoy curated sounds, custom menus, conversation cards, and romantic decor. In partnership with the dating app BLK and Black Restaurant Week, all platforms will share chef-favorite recipes on their social channels for those unable to attend in-person to prepare at home. Love Life is produced by Lionsgate Television and Feigco Entertainment and the second series debuts with three episodes on Oct. 28. Watch the trailer for Love Life season 2 on HBO Max above and check out more details on the restaurants featured on the Love Life Date Night and reservations here. Love Life Season 2 starts off on exactly the right foot with a captivating performance from its new main actor and an opening premise totally outside of the typical rom-com tropes. I absolutely cannot wait for the rest of the season. KENDRICK: Exactly. And I was really having a hard time. And I was like, do I not know this character as well as I should - you know, am I failing at my job, basically. 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D58 Rar Click Here ---> https://ssurll.com/2tkNPx Copyright: 2010 Sato et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The funders (CNRS, INSERM, Université de Strasbourg, ANR and the European Commission Structural Proteomics in Europe SPINE2-Complexes) had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Upon ligation to their cognate receptors, naturally-occurring vitamin A derivates mediate several physiological processes, such as vertebrate morphogenesis, cellular growth, differentiation or survival, as well as pathological conditions e.g premature birth, skin diseases or cancer development (reviewed in [1]). The atRA isomer binds exclusively to RARs whereas the 9-cis form of RA binds to both RARs and RXRs, (each of which exists as three isoforms α, β and γ) (reviewed in [2]). The ability of RAR/RXR to modulate the expression of target genes results from a combinatorial, coordinated and sequentially orchestrated exchange between nuclear hormone receptors (NHRs) and their coregulators. A general model of RAR/RXR-mediated transcription proposes that unliganded RAR/RXR heterodimers are bound to regulatory elements of their target genes and interact with transcriptional repressor complexes such as NCOR/SMRT/SIN3 to recruit histone deacetylases that lead to repression of target gene transcription [3]. Binding of agonist ligand to the nuclear receptor, triggers a conformational change in the ligand binding domain (LBD) with the repositioning of the C-terminal helix H12 creating a binding surface that allow coactivator to bind. Coactivator proteins such as CBP/p300, the p160 family, CARM1 or the Mediator contain one or more consensus LXXLL motifs that form an α-helix that fits into the hydrophobic cleft on the LBD to allow activation of target genes [4]. Antagonist ligands that prevent the C-terminal helix H12 from adopting its active conformation facilitate the interactions with corepressors. This new structure explains the RXR full antagonism activity of LG100754. The comparison with the previous RAR/RXR structures together with biochemical assays of the SRC-1 coactivator peptide recruitment to the heterodimer provides insight into the molecular mechanism of LG100754 action and explains its phantom effect. The crystal packing induces an intermolecular interaction between the flipped H12 of the RXRα-LG100754 and the coactivator binding surface of a symmetry related RXR molecule (Figure 4). This packing interface is made not only by the cofactor binding site but also H11 of RAR, H6, LoopH6-H7, H7 and H11 of RXR (Figure 4). Total surface area buried between the packing interface is 2000 Å2. Such tetrameric assembly induced by the flipped H12 is also observed in the other crystal structures of RXR [PDB IDs: 1LBD, 1G5Y, 1H9U, 2Q60, 2GL8] but the orientation of the present tetramer is different from any other. The oligomeric state of this complex in solution was determined by Small Angle X-ray Scattering (SAXS) the values of the radius of gyration Rg and of the maximal dimension Dmax as structural parameters (Table 1 and Figure S1). We further compared them to those of other RAR/RXR heterodimer or RXR homodimer or tetramer [19] and to theoretical values calculated from crystallographic structures. The values of Rg and of Dmax parameters measured for the RARα-atRA/RXRα-LG100754 complex clearly indicate that the complex is dimeric in solution. The Rg is 5 Å smaller than that calculated from the tetrameric crystal structure (Figure S1). Furthermore, the best fit of the experimental data is obtained unambiguously with the dimeric model in which one monomer is in a closed conformation and the other one in an open conformation with helix H12 pointing to the solvent (Figure S1). The experimental Rg is 1 Å larger than that of the heterodimer fully bound to agonists indicating a less compact conformation and 1 Å smaller than the relaxed apo-form of RXR dimer (Table 1). The tetramer generated by the crystallographic symmetry is induced by the highly concentrated conditions during the crystallization process. The binding mode of atRA (Figure 2B) to RARα in the present heterodimer is identical to that observed previously for RARα LBD [23]. The size of the atRA is 278 Å3. The comparison of the volume of the ligand binding cavity is 418 and 503 Å3 and the retinoic acid occupies 66.5% and 55.3% of the pockets for RARα and RARα, respectively. The difference of the cavity size around 100 Å3 is due to the different residues of the two isotypes forming the ligand binding pocket (LBP). The rexinoid antagonist LG100754 is buried in the LBP of RXRα formed by residues located on helices 3, 5, 7, 11 and the β-turn (Figure 5). The interactions are mainly hydrophobic with 80 Van Der Waals (VDW) contacts with the LBP at 4.2 Å cutoff. The carboxylate group makes an anchoring salt bridge with Arg321 [hArg316] (H5) and hydrogen bond with amino group of Ala332 [hAla327] (LoopH5-H6) in the hydrophobic pocket, similarly as observed with the carboxylate of 9-cisRA in the RXRα complex [24]. One water molecule makes a hydrogen bond network between the carboxyl group of LG100754 and the amino group of Leu314 [hLeu309]. The tetrahydronaphatalene moiety of LG100754 interacts with residues of H3, H5, H7 and H11 through VDW contacts and notably with Trp310 [hTrp305] (H5) (Figure 5). Compared to the 9-cisRA-bound RXR, the carboxylate and tetrahydronaphatalene group of LG100754 are located at the places which correspond to that of the carboxylate and β-ionone group of 9-cisRA. The propoxy group is pointing towards H11 and interacts with this helix through VDW contacts notably with Leu441 [hLeu436] which is repositioned (Figure 6A). The electron density map of the end of the propoxy group is poor because of its flexibility (see Figure 2B). A remarkable feature is the solvent accessibility of this LBP because of the flip of H12 to the solvent. According to crystallographic symmetry, this accessible region of the LBP is covered by LoopH11-H12 (mainly Phe443 [hPhe338] and Asp449 [hAsp444]) of another RXRα symmetry related molecule (Figure 4). The active agonistic conformation of H12 of RXRα is prevented by the long-tailed propoxy group of LG100754 which induces a steric hindrance with Leu456 [hLeu451], and consequently the coactivator peptide binding as shown for the superimposition of RXR-LG100754 and RXR-9cis RA (Figure 6A). Oleic acid, a neutral RXR ligand, has been crystallized in an RXR agonist conformation in RXR homodimer [25] and in an RXR antagonist conformation in RAR/RXR heterodimer [12]. Superposition of RXRα bound to LG100754 and to oleic acid in RXR antagonist conformation shows two different antagonist conformations. Indeed, the propoxy group of LG100754 induces a steric hindrance with Leu446 [hLeu441] in the LoopH11-H12 as observed in the RXR-oleic acid antagonist conformation, precluding H12 binding to the coactivator cleft (Figure 6B). This new structural information is in agreement with the inability of RXRα-LG100754 homodimer to bind to any coactivator or corepressor [26]. Phe442 [hPhe437] and Phe443 [hPhe438] in H11 of RXRα which are known to play important roles in the transition of the apo to agonist conformation [13], flip out to the solvent region in the present antagonistic structure (Figure 6). (A) Close-up view showing the superposition of RXRα LBP bound to LG100754 (in cyan) and to 9-cisRA (in red). LG100754 and 9-cisRA are shown by stick representation in yellow and magenta, respectively, with oxygen atoms in red. The propoxy group of LG100754 induces a steric hindrance (solid arrow) with Leu456 (H12). Residues involved in the transition agonist to antagonist transition (dotted arrow) conformation are labelled. (B) Close up view of the superposition of RXRα LBP bound to LG100754 (in cyan) and to oleic acid in antagonist conformation (in orange). Oleic acid is show by stick representation in magenta. The propoxy group of LG100754 induces a steric hindrance (solid arrow) with Leu446 as shown by an arrow. In contrast, the structural basis of the antagonism of UVI3003 should be similar to that of LG100754. The crystal structure of the complex of RXR and the partial agonist UVI3002 [30] (Figure 1) reveals that the alkyl ether group of UVI3002 is located at the same position as the propoxy group of LG100754 but its length do not prevent the agonist conformation. Therefore, UVI3003 which has a longer alkyl group than UVI3002 should similarly prevent H12 associating to the LBD and the RXR complex should adopt an antagonistic conformation as in RXR-LG100754. In agreement with this molecular mechanism of antagonism, analogues of LG100754 with shorter groups such as ethyl or methyl groups instead of the propoxy group act either as partial agonist or full agonist for RXR, respectively [31]. A recent NMR study on the effect of RXR antagonists on the conformation of H12 in the RXR homodimer and in the permissive PPAR/RXR with PPAR bound to an agonist ligand reveals similar features, namely the rexinoid antagonist is unable to stabilize a compact state and therefore prevents the coactivator from binding to RXR [32]. To quantify the recruitment of the SRC-1 NR2 peptide to RARα, RXRα and RARα/RXRα, Isothermal Titration Calorimetry (ITC) was used, thus providing the full thermodynamic profile of SRC-1 binding. The similar SRC-1 NR2 peptide (25 residues) to the one used in ESI-MS was used in the ITC experiments. Representative titrations for SRC-1 NR2 binding are shown in Figure S3. In the presence of LG100754, the RARα monomer binds the SRC-1 NR2 peptide with an affinity similar to that measured in presence of an RAR agonist ligand (Figure 9 and Table S2). Therefore, the LG100754 ligand stabilizes the agonist conformation of RAR that renders accessible the binding surface for coactivator recruitment. Docking of L100754 in the ligand binding pocket of hRARα reveals that the ligand easily adapts to fit the RARα agonist conformation without significant steric clashes (Figure S4). No significant interaction of the SRC-1 NR2 peptide with RXRα LBD was observed (Figure S3) in agreement with our ESI-MS data and with the literature [26]. In RAR/RXR LBDs bound to LG100754, SRC-1 NR2 binds to the heterodimer with a stoechiometry of one peptide per heterodimer and with an affinity similar to the one for the RARα monomer. Together these data demonstrate (Figure 9) that the LG100754 compound is able to stabilize an agonist conformation when bound to RAR while in RXR it inhibits the interaction with the coactivator SRC-1 peptide. LG100754 has also been shown to be able to dissociate corepressors from RAR [16] and to prevent their binding to RXR [26]. 59ce067264 https://www.ubondedu.com/forum/untitled-category-10/married-life

Hand Book-scert Kerala CLICK HERE - https://cinurl.com/2tkMxq With a detailed syllabus in hand, candidates can interlink concepts and study, which is a good tactic for sound preparation in addition to the clarity of concepts. Experts with years of experience and even toppers over the years highlight the importance of syllabus and credit an in-depth understanding of the syllabus to their performance. Thiruvananthapuram: Special handbook including the Malayalam alphabet will be distributed among students from classes 1 to 4 during the coming academic year. The preparation of the handbook is under consideration as the first volume of the textbooks has been printed and distributed to schools and the design of the second volume has been completed. The State Council of Educational Research and Training (SCERT), Kerala which duly functions as an R&D institute at the state level provide valuable guidance, support and assistance to the State Education Department, for its effort in improving the quality of education. To accomplish the real goal, the SCERT develops instructional materials for teachers at all levels. As the quality of teaching forms the best part of education, teachers are given training along with supplying supporting materials like teachers' handbooks and other academic books. With the changing system of learning process, teachers must also adapt to the environment giving the best support to the students' community. The numerical expansion of schooling and the rising need of quality education triggered the modification in the curriculum and teaching method. An innovative system of continuing education for teachers would benefit the learners to gather more information and even develop their abilities. The SCERT plays an important role in educational research and training by undertaking programs for quality improvement in school education. SCERT Kerala teachers handbookTo cater the need of the students, educators should be well versed with the subject. The SCERT Kerala teachers handbooks of subjects like English, Malayalam, Hindi, Mathematics, Science and Social Science of class 1, 3, 5 and 7 are now available at the official website. These handbooks have been designed by experts from the relevant fields. Teachers can best use these resources to make the classroom live and interesting. Written in simple language, the handbooks would enable the teachers to plan each lesson while the students could be able to gather more knowledge. Each lesson is well structured and would keep the learner acquire information more than the typical teaching method. As each chapter is illustrated with pictorial examples, the teacher could be able to channelize the acquired knowledge to the learners too. All the teachers in the government circle can use these learning materials to imbibe knowledge to the present generation.Get SCERT Kerala teachers handbook 2014 59ce067264 https://www.alwayshavethyme.com/forum/get-started-with-your-forum/we-the-best-forever-zip

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